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 可溶性高级糖基化终末产物受体sRAGE
可溶性高级化糖基化终末产物受体sRAGE可以结合高级糖基化终末产物（AGE），是反映二型糖尿病或代谢综合征微血管损伤一个可靠的生物标示物，与IMT(intima-media-thickness)有显著的正相关关系。可溶性高级化糖基化终末产物受体在体内存在一种变异型缺乏转膜区结构，它可以分泌到血液中， 称之为内源性分泌型高级化糖基化终末产物受体esRAGE。目前研究资料表明，可溶性高级化糖基化终末产物受体sRAGE含量较高，是内源性分泌型糖基化高级化终末产物受体esRAGE的5~10倍高。可溶性高级化糖基化终末产物受体sRAGE和IMT相关。而esRAGE只和年龄相关。爱迪博生物的研究人员关注于研究可溶性高级化糖基化终末产物受体sRAGE在代谢综合征中的生物学意义，它在急性冠状动脉综合征伴有血管损伤的临床检测意义值得关注。研究资料显示，血清或血浆中的sRAGE在慢性肾脏疾病、冠心病、二型糖尿病、肥胖等呈现下降趋势， 并且与BMI、CRP、甘油三酯、低密度脂蛋白呈现负相关关系。与高密度脂蛋白、脂联素等呈现正相关关系。提示， sRAGE是机体内的一种保护性细胞因子。

RAGE and cellular stress: initiating factor and/or amplifier of inflammatory mechanisms and tissue injury? In the multiple hit model, certain disease states are characterized by basally enhanced generation of RAGE ligands (AGEs, S100/calgranulins, amphoterin, and/or Mac-1), in parallel with increased expression of RAGE (hit one). On superimposed stresses (hit two), such as infection, renal failure, hyperglycemia, physical stress, hyperlipidemia, aging, or autoimmunity, further generation of RAGE ligands activates signal transduction mechanisms, via RAGE, that lead to upregulation of inflammatory and prothrombotic pathways. If left unchecked, these processes amplify inflammation and tissue destruction. In the alternate view, basal upregulation of the ligand–RAGE axis itself, as a single hit, contributes innately to the development of vascular inflammation and tissue injury. Experiments must be performed to dissect the contribution of each facet of the RAGE axis in vascular and other forms of inflammatory disorders.

Model for the potential sources of sRAGE. There are a number of potential means by which sRAGE may be generated. First, it is possible that yet to be delineated mechanisms may cleave sRAGE from the endogenous full-length cell surface receptor. Second, multiple discrete novel splice variants of RAGE may generate diverse sRAGEs. The assay used by Falcone and colleagues in this issue of Arteriosclerosis, Thrombosis, and Vascular Biology does not distinguish these possibilities. Although this cartoon suggests that endothelial cells may be a principal source of sRAGE, it is possible that other cells such as smooth muscle cells or circulating inflammatory cells may generate sRAGEs. Key roles for sRAGEs, as decoys for the cell surface receptor RAGE, may serve as biomarker and/or endogenous protection factor in diseases characterized by upregulation of RAGE ligands.

说明书

可溶性高级糖基化终末产物受体 (人） 酶联免疫试剂盒
产品号 SK00112-02
标准曲线范围: 62.5 - 4000 pg/mL
检测灵敏度 30 pg/mL
检测时间 4小时10分钟
样本 seum
样本体积 100 ul
正常血清值 300 -4500 pg/ml
正常血清值 1335 (936–1954) pg/ml
二型糖尿病 601.8 ± 477.3 pg/ml
冠心病 966 (658–1372) pg/ml
样本稀释 2 x
批内误差 4.7%
批间误差 6.0%
交叉反应 human sRAGE100%

可溶性高级糖基化终末产物受体 (大鼠） 酶联免疫试剂盒
产品号 SK00112-03
标准曲线范围: 128 - 80000 pg/mL
检测灵敏度 300 pg/mL
检测时间 16小时
样本 seum
样本体积 50 ul
正常血清值 3000 - 8000 pg/ml
样本稀释 4 x
批内误差 4%
批间误差 8%
交叉反应 大鼠或小鼠 sRAGE100%

 

可溶性高级糖基化终末产物受体 (小鼠） 酶联免疫试剂盒
产品号 SK00112-04
标准曲线范围: 125 - 8000 pg/mL
检测灵敏度 60 pg/mL
检测时间 4小时10分钟
样本 seum
样本体积 100 ul
血清值 400 -4800 pg/ml
样本稀释  
批内误差 5.2%
批间误差 8.0%
交叉反应 小鼠 sRAGE100% ，大鼠sRAGE20%

 

 

 

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试剂盒名称 目录号 规格
价格
可溶性高级糖基化终末产物受体sRAGE样本检测服务 S00112-02 80
询价
可溶性高级糖基化终末产物受体sRAGE（人）酶联免疫试剂盒 SK00112-02 96T
询价
可溶性高级糖基化终末产物受体sRAGE（大鼠）酶联免疫试剂盒 SK00102-03 96T
询价
可溶性高级糖基化终末产物受体sRAGE（小鼠）酶联免疫试剂盒 SK00102-04 96T
询价
重组可溶性高级糖基化终末产物受体sRAGE(人） 00112-02-100 100 ug
询价
抗人sRAGE 免疫组化抗体 ADB-AF1145 100 ul
询价
重组可溶性高级糖基化终末产物受体sRAGE(大鼠） 00112-03-100 100 ug
询价
重组可溶性高级糖基化终末产物受体sRAGE(小鼠） 00112-04-100 100 ug
询价
抗小鼠RAGE免疫荧光 抗体 ADB-AF1179 100 ul
询价
小鼠抗人sRAGE 单抗(用于蛋白印迹和受体阻断） ADB-MAB11451 100 ug
询价
 
 

Homozygous RAGE null mice display decreased neointimal expansion on acute femoral artery endothelial denudation. Homozygous RAGE null mice and wild-type littermates were subjected to femoral artery guide wire injury. Intima/media ratio was determined on day 28 after injury (A). Van Gieson’s elastic stain was performed on a representative femoral artery section from a wild-type mouse (B) and a RAGE null mouse (C). Scale bar=50 μm. Shi Fang Yan et al. Circulation Research. 2003;93:1159.

Immunohistochemistry (x5) (A) and Western blot (B) for RAGE in diabetic or nondiabetic plaques, and relationship between glycemic control and RAGE expression in diabetic plaques (C). Note the immunohistochemistry at high magnification (x63) (A, box) showing prevalent RAGE localization in perivascular macrophages in diabetic plaques. These results are typical of 30 diabetic and 30 nondiabetic plaques. Circulation. 2003;108:1070

Confocal microscopy showed staining for RAGE (green staining) on plaque-derived macrophages, concomitant (red staining) with CD68, COX-2, MMP-2, and MMP-9 expression. These results are typical of 30 diabetic and 30 nondiabetic plaques.
 

 

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