Serpin E1, also known as Plasminogen Activator Inhibitor-1 (PAI-1), is a member of the serpin superfamily of serine protease inhibitors . Serpin E1 is the primary inhibitor of urokinase-type and tissue-type plasminogen activators (uPA and tPA), which convert plasminogen to plasmin. The PA-plasmin system is involved in multiple physiological and pathological processes such as fibrinolysis, fibrosis, angiogenesis, wound healing, and tumor cell invasion and metastasis . For example, high levels of uPA and Serpin E1 correlate with poor prognosis in patients with breast cancer, and uPA and Serpin E1 are among the most reliable biomarkers whose clinical values were confirmed in level 1 evidence (LOE-1) studies . Besides breast cancer, Serpin E1 has been linked to many diseases such as the metabolic
syndrome, asthma, major depressive disorder (MDD), and aging-related pathologies. Absence of Serpin E1 in the plasma causes abnormal bleeding, and high plasma levels of Serpin E1 are associated with inherited thrombophilia . The deduced amino acid sequence of human Serpin E1 is 99.5%, 86.1%, 80.8%, and 78.6% identical to that of chimpanzee, canine, rat, and mouse, respectively. Synthesized and secreted by many cell types, free Serpin E1 is relatively unstable in its active form and readily converts into a latent, inactive form by spontaneous insertion of its reactive center loop (RCL) into the -sheet core of the protein . Binding of vitronectin to Serpin E1, however, stabilizes the active form by preventing the conformation change and also broadens the target specificity of Serpin E1 beyond uPA and tPA . The current model for the Serpin E1- vitronectin complexes involves the formation of a 2:1 intermediate and a few higher order
complexes . The active form of Serpin E1 binds tightly with uPA and tPA in a 1:1 ratio. After the formation of an initial docking (Michaelis) complex, the proteases cleave the RCL of Serpin E1 to form a stable, covalent complex, resulting in the inactivation of the targeted protease .
The Quantikine Serpin E1/PAI-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Serpin E1 in cell culture supernates, cell lysates, and plasma. It contains Sf 21-expressed recombinant human Serpin E1 and has been shown to accurately quantitate the recombinant factor in its active and latent forms as well as in the vitronectin complexes. However, the kit does not detect recombinant human Serpin E1 in complexes with recombinant
human uPA and recombinant human tPA. Results obtained using natural human Serpin E1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that the Quantikine Serpin E1/PAI-1 kit can be used to determine relative mass values for naturally occurring Serpin E1.

人PAI-1 酶联免疫试剂盒 [SK00210-01]可以检测血浆的样本。血浆含量在 0.87 ~ 19 ng/ml; 灵敏度（Sensitivity ）： 0.12 ng/ml； 标准曲线范围是0.312- 20 ng/ml。SK00210-01试剂盒需要样本体积100微升；

人PAI-1 酶联免疫试剂盒 [SK00210-06]可以检测血浆的样本。血浆含量在 0.87 ~ 19 ng/ml; 灵敏度（Sensitivity ）： 0.12 ng/ml； 标准曲线范围是0.312- 20 ng/ml。SK00210-06试剂盒需要样本体积50微升；

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