Plasma matrix metalloproteinase-9 and left ventricular remodelling after acute myocardial infarction in man: a prospective cohort study. Eur Heart J. 2007 March; 28(6): 711–718.
| 产品号 |
SK00160-01 |
| 标准曲线范围: |
0.312 ~ 20 ng/ml |
| 检测灵敏度 |
0.156 ng/ml |
| 样本体积 |
100 ul |
| 样本稀释 |
血清、唾液、尿液和细胞培养液100倍 |
| |
血浆是40倍稀释 |
| 批内误差 |
2.9% |
| 批间误差 |
7.9% |
| 交叉反应 |
human MMP-9 100% |
| 样本检测 |
血清:169 - 705 ng/ml |
| 样本检测 |
血浆:13.2 - 105 ng/ml |
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产品目录号:SK00160-02

ELISA Protocol
| 产品目录号 |
SK00160-02 |
| 标准曲线范围: |
0.030 ~ 2 ng/ml |
| 检测灵敏度 |
20 pg/ml |
| 样本体积 |
100 ul |
| 样本稀释 |
血清、唾液、尿液和细胞培养液500倍 |
| |
血浆是100倍稀释 |
| 批内误差 |
4.9% |
| 批间误差 |
5.9% |
| 交叉反应 |
human MMP-9 100% |
| 样本检测 |
血清:169 - 705 ng/ml |
| 样本检测 |
血浆:13.2 - 105 ng/ml |
—————————————————————————————————
|
| 标准曲线范围: 0.078 ~5 ng/ml |
| 检测灵敏度:0.007 ng/ml |
| 样本体积:50微升 |
| 样本稀释:血清需要50倍;血浆需要40倍 (加入稀释后的样本浓度仍高于25ng/ml, 可能还要继续稀释一定的倍数) |
| 批内误差:5.1~8.4% |
| 批间误差:7.8~9.6% |
| 交叉反应:小鼠的MMP-9是100%;与人或大鼠的不交叉 |
| 样本检测:血清 (n=20)121 +- 44 ng/ml |
| 样本检测:血浆 (EDTA, n=20) 59 +- 48 ng/ml |

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|
| 标准曲线范围: |
0.312 ~ 20 ng/ml |
| 检测灵敏度 |
0.013 ng/ml |
| 样本体积 |
100 ul |
| 样本稀释 |
血清、唾液、尿液和细胞培养液100倍 |
| |
血浆是40倍稀释 |
| 批内误差 |
4.1% |
| 批间误差 |
7.6% |
| 交叉反应 |
与人MMP-9或NGAL无交叉反应 |
| 样本检测 |
血清:8.7 - 164 ng/ml |
| 样本检测 |
血浆:1.92 - 44.8 ng/ml |
| 样本检测 |
唾液:7.38 - 109 ng/ml |
|
| NGAL, also known as lipocalin-2, is a member of the lipocalin family that is comprised of functionally diverse but structurally conserved small proteins . NGAL has been implicated in a variety of processes including inflammation, apoptosis, and organogenesis, and is a promising biomarker for acute renal failure . NGAL biosynthesis is stimulated by the Toll-like receptors on immune cells upon bacterial invasion; secreted NGAL then limits bacterial growth by sequestrating the iron-laden siderophore. NGAL exists as monomer (25 kDa), homodimer, and disulfide bond-linked heterodimer with MMP-9. The MMP-9/NGAL complex (125 kDa) is one of several MMP forms with high molecular weights (> 100 kDa) detected in the urine of cancer patients . NGAL is proposed to modulate MMP-9 activity by protecting it from degradation. |
| 电子信箱 |
Info@AdipoBiotech.com |
| 电话号码 |
010-81786624; 81786244 |
|