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胶质细胞源性神经营养因子GDNF (Glial cell line-Derived Neurotrophic Factor)

 

HumanGDNF : 211 AA， MW 23720 Da
Signal sequence: 1-19, Pro-BDNF: 20-75, Mature BDNF: 78-211 
        10         20         30         40         50         60   
MKLWDVVAVC LVLLHTASAF PLPAGKRPPE APAEDRSLGR RRAPFALSSD SNMPEDYPDQ    
        70         80         90        100        110        120   
FDDVMDFIQA TIKRLKRSPD KQMAVLPRRE RNRQAAAANP ENSRGKGRRG QRGKNRGCVL   
       130        140        150        160        170        180   
TAIHLNVTDL GLGYETKEEL IFRYCSGSCD AAETTYDKIL KNLSRNRRLV SDKVGQACCR   
      190        200        210   
PIAFDDDLSF LDDNLVYHIL RKHSAKRCGC I 211
Swiss-Prot entryP39905;
人胶质细胞源性神经营养因子(GDNF)酶联试剂盒
名称 人胶质细胞源性神经营养因子(GDNF)酶联试剂盒
产品号 SK00029-01
检测范围 15.6- 1000 pg/mL
灵敏度 2 pg/mL
样本类型 血浆、血清
样本体积 100 ul
稀释倍数  
批内差异 4%
批间差异 6%
特异性 100% with rh GDNF
Cell surface receptors for GDNF family ligands and neurotrophins in the peripheral nervous system
.?Communication of GDNF family ligands with their receptors. Homodimeric GDNF family ligands: GDNF (green), neurturin (red), artemin (blue) and persephin (yellow) activate the transmembrane Ret tyrosine kinase (beige) by binding with high affinity to different GPI-linked GFRα receptors. The complete lines represent preferred functional binding: GDNF specifically binds to GFRα1 (green), neurturin to GFRα2 (red), artemin to GFRα3 (blue) and persephin to GFRα4 (yellow). Interactions shown by dotted lines may not be significant in vivo. Mammalian GFRα4 is shorter than other GFRα receptors, becasuse it lacks the N-terminal globular domain. Binding of ligand-GFRα complex to Ret triggers its homodimerization, phosphorylation and intracellular signalling. For GDNF, an alternative model exists, where GDNF binds to a pre-existing GFRα1–Ret complex. GDNF can trigger Ret-independent signalling through GFRα1.

 

 

Expression of neurotrophic factors in cerebrospinal fluid and plasma of children with viral and bacterial meningoencephalitis
AIM: To evaluate the expression of neurotrophic factors (nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), glial-derived neurotrophic factor (GDNF)) and their association with the clinical-radiological characteristics and outcome of children with viral and bacterial meningoencephalitis (ME). METHODS: Prospective observational clinical study performed on 13 children with ME and 12 controls with non-inflammatory obstructive hydrocephalus. Neurotrophic factor levels in the cerebro-spinal fluid (CSF) and plasma were measured using an immunoenzymatic assay. RESULTS: High levels of NGF and BDNF were demonstrated in all patients, while GDNF levels did not undergo significant variations. NGF expression in the CSF was higher in viral ME than in bacterial ME and was correlated with CSF cellularity (particularly mononuclear cells). BDNF expression in the CSF was higher in bacterial ME than in viral ME and was correlated with CSF cellularity and blood platelet count. No relationships were noted between CSF protein or serum C-reactive protein levels and the expression of neurotrophic factors. Regarding clinical and radiological features, elevated NGF/BDNF levels in the CSF correlated with higher incidence of seizures and prolonged comatose state and with specific radiological lesions. No correlation was found between NGF/BDNF levels and final outcome. CONCLUSIONS: The variations in neurotrophic factor levels may reflect an endogenous attempt at neuroprotection against biochemical and molecular changes during both viral and bacterial ME. The expression of these factors is likely to play a neuro-immunomodulatory or neurosurvival role in ME infections.
Chiaretti A,., et al. Acta Paediatr. 2004 Sep;93(9):1178-84.

 

 
产品名称
目录号
规格
价格
人胶质细胞源性神经营养因子(GDNF)酶联试剂盒
SK00029-01
96 T
3500
人胶质细胞源性神经营养因子(GDNF)酶联试剂盒
SK00029-02
96 T
6500

 

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