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Insulin Rabbit Ab CS4590
Applications Key: IHC-P=Immunohistochemistry (Paraffin) IF-F=Immunofluorescence (Frozen) F=Flow Cytometry Specificity / SensitivityInsulin Antibody detects endogenous levels of total insulin protein. Source / PurificationPolyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human insulin. Antibodies are purified by protein A and peptide affinity chromatography. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded rat pancreas, showing staining of β-cells using Insulin Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse pancreas, showing staining of β cells, using Insulin Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human pancreas, showing staining of β cells, using Insulin Antibody. Flow Cytometry
Flow cytometric analysis of untreated β-TC cells, using Insulin Antibody (blue) compared to a nonspecific negative control antibody (red).
Insulin (C27C9) Rabbit mAb
Applications Key: IHC-P=Immunohistochemistry (Paraffin) IF-F=Immunofluorescence (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry Specificity / SensitivityInsulin (C27C9) Rabbit mAb detects endogenous levels of total insulin protein. Source / PurificationMonoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human insulin. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human pancreas, showing the staining of β cells, using Insulin (C27C9) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical anaylsis of paraffin-embedded mouse pancreas, showing staining of β cells, using Insulin (C27C9) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded rat pancreas, showing staining of β cells, using Insulin (C27C9) Rabbit mAb. Flow Cytometry
Flow cytometric analysis of HeLa cells (blue) and β-TC-6 cells (green) using Insulin (C27C9) Rabbit mAb. IF-IC
Confocal immunofluorescent analysis of β-TC-6 and C2C12 cells using Insulin (C27C9) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). IF-IC
Confocal immunofluorescent analysis of rat pancreas using Insulin (C27C9) Rabbit mAb (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). BackgroundThe maintenance of glucose homeostasis is an essential physiological process that is regulated by hormones. An elevation in blood glucose levels during feeding stimulates insulin release from pancreatic β cells through a glucose sensing pathway (1). Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to its secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Insulin stimulates glucose uptake from blood into the skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus (2).
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