脂肪酸结合蛋白-4(Fatty Acid Binding Protein-4)和代谢综合症

脂肪酸结合蛋白-4FABP4，又叫脂肪细胞型脂肪酸结合蛋白（Adipocyte FABP), 属于 FABP 家族成员。 它有132个氨基酸残基，人和小鼠的氨基酸同源性是91%。 脂肪酸结合蛋白-4主要的表达合成部位是脂肪细胞和巨嗜泡沫细胞。其主要功能是作为脂质转运蛋白结合长链脂肪酸和维甲酸（视黄酸）。近年研究资料表明， 脂肪酸结合蛋白-4FABP4过表达，血浆含量增加可以反映代谢综合征的一个可靠生物标示物。
脂肪酸结合蛋白-4的检测可以通过酶联免疫、蛋白质印迹试剂盒。可以检测血清或血浆的样本。检测时样本可能需要稀释1-2倍。

Circulating adipocyte-fatty acid binding protein levels predict the development of the metabolic syndrome: a 5-year prospective study

BACKGROUND: Adipocyte-fatty acid binding protein (A-FABP), a major cytoplasmic protein in adipocytes, plays a central role in the development of diabetes and atherosclerotic cardiovascular disease in experimental animals. We have previously shown that A-FABP is present in the bloodstream and that its circulating levels correlate with metabolic risk factors in a cross-sectional study. In the present study, we further evaluated the prospective association of A-FABP with the metabolic syndrome (MetS) as defined by the updated National Cholesterol Education Program criteria. METHODS AND RESULTS: In the present study, 495 nondiabetic adults from the population-based Hong Kong Cardiovascular Risk Factor Prevalence Study were prospectively followed up for 5 years. The relationship of serum A-FABP with the MetS and its components was investigated. At baseline, high A-FABP levels were associated with the MetS (odds ratio, 4.0; 95% CI, 1.5 to 10.4; highest versus lowest sex-specific tertile, adjusted for age, body mass index, the homeostasis model assessment index for insulin resistance, C-reactive protein, and adiponectin, P=0.005). On long-term follow-up, subjects with higher baseline A-FABP levels had progressively worse cardiometabolic risk profile and increasing risk of the MetS. Among 376 subjects without the MetS at baseline, 50 had developed it at 5 years. Apart from the homeostasis model assessment index for insulin resistance (P=0.001), baseline A-FABP was the only independent predictor of the development of the MetS during the 5-year follow-up (odds ratio, 4.7; 95% CI, 1.8 to 11.9; highest versus lowest sex-specific tertile, P=0.001, adjusted for the homeostasis model assessment index for insulin resistance and body mass index). A-FABP was predictive of the MetS even after adjustment for each of its individual components. CONCLUSIONS: Circulating A-FABP predicts the development of the MetS independently of adiposity and insulin resistance.

Xu A, et al. Circulation. 2007 Mar 27;115(12):1537-43

Human AFABP / FABP-4 QCRat AFABP / FABP-4 QC

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蛋白质印迹检测大鼠脂肪组织的脂肪酸结合蛋白4使用小鼠抗人脂肪酸结合蛋白4多克隆抗体，抗体说明书

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蛋白质印迹检测大小鼠脂肪组织的脂肪酸结合蛋白4使用兔抗人脂肪酸结合蛋白4多克隆抗体，抗体说明书

免疫组化检测人腹腔白色脂肪组织使用兔抗脂肪型脂肪酸结合蛋白抗体

 

免疫组化检测大鼠腹腔白色脂肪组织使用兔抗人脂肪型脂肪酸结合蛋白抗体