Adipose Differentiation-Related Protein (ADRP)

ADRP/ADFP, the major lipid droplet protein, is capable of sequestering TG in the cytosol, diverting it from entering into ER lumen for VLDL secretion. ADFP is involved in LD formation and/or maturation. Macrophages play an important role in atherosclerosis. An earlyevent in atherosclerosis is the accumulation of LDs in lesionalmacrophages associated with ADFP accumulation. ADFP is the most abundant LD-associated protein found in these cells. Modified lipoproteins, e.g., oxidized LDL or acetylated LDL, that arehighly atherogenic upregulate Adfp expression in macrophagesin vitro . Furthermore, Adfp mRNA is upregulated in human atherosclerotic plaques compared with lesion-free areas of thesame arteries . Conversely, ADFP overexpression in THP-1macrophage enhances lipid accumulation and prevents lipid efflux.These results suggest that ADFP is potentially a proatherosclerogenic protein. Our preliminary study using Adfp-deficient mice seems to support this notion. ADFP is the predominant LD-associated protein in skeletal musclein humans . Furthermore, muscle ADFP is lower in insulin-resistant subjects, a situation that can be reversed by weight reduction or by troglitazone treatment coincident with an improvement in glucose tolerance. It is possible that the upregulation of ADFP may help sequester fatty acids as TG in LDs, protecting the muscle from the detrimental effects of fatty acids on insulinaction and glucose homeostasis . Chang BHJ et al. Am J Physiol Gastrointest Liver Physiol 292: G1465-G1468, 2007

Synonyms

• Adipophilin
• ADFP

Human ADRP, 437 amino acids, MW 48074 Da 10 20 30 40 50 60 MASVAVDPQP SVVTRVVNLP LVSSTYDLMS SAYLSTKDQY PYLKSVCEMA ENGVKTITSV 70 80 90 100 110 120 AMTSALPIIQ KLEPQIAVAN TYACKGLDRI EERLPILNQP STQIVANAKG AVTGAKDAVT 130 140 150 160 170 180 TTVTGAKDSV ASTITGVMDK TKGAVTGSVE KTKSVVSGSI NTVLGSRMMQ LVSSGVENAL 190 200 210 220 230 240 TKSELLVEQY LPLTEEELEK EAKKVEGFDL VQKPSYYVRL GSLSTKLHSR AYQQALSRVK 250 260 270 280 290 300 EAKQKSQQTI SQLHSTVHLI EFARKNVYSA NQKIQDAQDK LYLSWVEWKR SIGYDDTDES 310 320 330 340 350 360 HCAEHIESRT LAIARNLTQQ LQTTCHTLLS NIQGVPQNIQ DQAKHMGVMA GDIYSVFRNA 370 380 390 400 410 420 ASFKEVSDSL LTSSKGQLQK MKESLDDVMD YLVNNTPLNW LVGPFYPQLT ESQNAQDQGA 430 EMDKSSQETQ RSEHKTH 437 Swiss-Prot entry Q99541; ADRP is the major lipid droplet protein. May be involved in development and maintenance of adipose tissue.

产品名称	目录号	规格	价格
Human Adipocyte Differentiation-Related Protein (ADRP)	00011-01-100	100 ug	询价
人脂肪细胞分化相关蛋白	00011-01-100	100 ug	询价

 

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Inhibition of ADRP prevents diet-induced insulin resistance

Diets with high fat content induce steatosis, insulin resistance and type 2 diabetes. The lipid droplet protein, Adipose Differentiation-Related Protein (ADRP), mediates hepatic steatosis but whether this affects insulin action in the liver or peripheral organs in diet-induced obesity is uncertain. We fed C57BL/6J mice a high-fat diet and simultaneously treated them with an antisense oligonucleotide (ASO) against ADRP for 4 weeks. Glucose homeostasis was assessed using clamp and tracer techniques. ADRP ASO decreased the levels of triglycerides and diacylglycerol in the liver, but fatty acids, long chain fatty acyl CoAs, ceramides and cholesterol were unchanged. Insulin action in the liver was enhanced following ADRP ASO treatment, whereas muscle and adipose tissue were not affected. ADRP ASO increased the phosphorylation IRS1, IRS2 and Akt, and decreased gluconeogenic enzymes and PKC, consistent with its insulin sensitizing action. These results demonstrate an important role for ADRP in the pathogenesis of diet-induced insulin resistance.

Varela G.M. et al. Am J Physiol Gastrointest Liver Physiol (July 31, 2008). doi:10.1152/ajpgi.90204.2008