Human Corin ELISA Kit

Circ Heart Fail. 2010 Jan 8. [Epub ahead of print]

Plasma Soluble Corin in Patients with Heart Failure.

BACKGROUND: -Corin is a transmembrane protease that processes natriuretic

peptides in the heart. Like many membrane proteins, corin is shed from the cell

surface. METHODS AND RESULTS: -In this study, we obtained plasma samples from

healthy controls and patients with heart failure (HF) and acute myocardial

infarction (AMI). Soluble corin levels in plasma were measured by an ELISA

method. In healthy adults (n=198), plasma corin levels were 690 (SD 260) pg/mL.

The corin levels did not differ significantly among different age groups. In

patients with HF (n=291), plasma corin levels were significantly lower compared

to that of healthy controls (365 (SD 259) pg/mL, p<0.001). The reduction in

plasma corin levels appeared to correlate with the severity of HF. In patients of

New York Heart Association (NYHA) classes II, III and IV, plasma corin levels

were 450 (SD 281) (n=69), 377 (SD 270) (n=132), and 282 (SD 194) (n=90) pg/mL,

respectively (p<0.001 class II vs. IV; p<0.05 class III vs. IV). In contrast,

plasma corin levels in patients with AMI (n=73) were similar to that of healthy

controls (678 (SD 285) pg/mL, p>0.05). CONCLUSIONS: -Soluble corin was detected

in human plasma. Plasma corin levels were reduced significantly in patients with 

HF but not AMI. Our results indicate that corin deficiency may contribute to the 

pathogenesis of HF and that plasma corin may be used as a biomarker in the