Osteocalcin, a major non-collagenous bone protein, is synthesized by osteoblasts during bone formation. Most of the osteocalcin is incorporated into the bone matrix and a small amount is secreted into the circulation. Studies have shown that serum osteocalcin levels are directly related to osteoblastic activity and bone formation.
The Mouse Osteocalcin EIA kit is a sandwich ELISA assay. Two mouse osteocalcin antibodies are employed, each directed toward an end (C or N terminal) of the osteocalcin molecule. One mouse osteocalcin antibody is bound to the wells which binds the mouse osteocalcin standard or sample, the other biotinylated antibody completes the sandwich.
This sandwich ELISA kit is specific for intact mouse osteocalcin only. Both carboxylated and decarboxylated mouse osteocalcin are recognized. Mouse Osteocalcin can be measured at a 1:10 to 1:20 dilution from serum or heparinized plasma. Bone extracts and serum-free cell culture supernates can be measured directly. This kit shows good correlation (r=0.90) with the BTI mouse osteocalcin RIA.
The protocol has an overnight incubation and yields a
1-50ng/ml range. As little as 5-10 microliters of mouse serum is required for duplicate determinations. Each kit contains all the necessary reagents, precoated 96-well strips and a complete protocol.
Sample Size: 100ul (diluted serum)
Assay Time: 5 hours or overnight
Sensitivity: 0.5 ng/ml
Range: 0.78-50 ng/ml or 0.33-20 ng/ml
Intraassay Variation: 4%
Interassay Variation: 7%
The assay can be run either overnight (0.33-20ng/ml) or in 6 hours (0.78-50ng/ml). As little as 10 microliters of rat serum is required for duplicate determinations. Each kit contains all the necessary reagents, an authentic rat serum control, precoated 96-well strips and a complete protocol.
产品家族搜索-Family Search：