The Rat Osteocalcin EIA Kit is a sandwich ELISA assay. Two osteocalcin antibodies are employed, each directed toward an end of the (C or N terminal) osteocalcin molecule. The N-terminal monoclonal antibody is bound to the wells and when reacted with rat osteocalcin standard or sample, the C-terminal rat osteocalcin antibody completes the sandwich. This ELISA kit shows good correlation (r = 0.95) with our rat osteocalcin RIA.
This sandwich ELISA kit is specific for rat osteocalcin only. Both carboxylated and decarboxylated rat osteocalcin are recognized. Rat osteocalcin can be measured directly from serum, heparinized plasma, bone extracts and serum-free cell culture supernates.
Specifications:
Sample Size: 100ul (diluted serum)
Assay Time: 5 hours or overnight
Sensitivity: 0.5 ng/ml
Range: 0.78-50 ng/ml or 0.33-20 ng/ml
Intraassay Variation: 4%
Interassay Variation: 7%
The assay can be run either overnight (0.33-20ng/ml) or in 6 hours (0.78-50ng/ml). As little as 10 microliters of rat serum is required for duplicate determinations. Each kit contains all the necessary reagents, an authentic rat serum control, precoated 96-well strips and a complete protocol.
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