Osteocalcin (5800 daltons) is a specific product of the osteoblast. Only a small amount of osteocalcin is released directly into the circulation, it is primarily deposited into the bone matrix. Studies have shown that osteocalcin circulates both as the intact (1-49) protein and as N-terminal fragments. The major N-terminal fragment is the peptide (1-43).
The Mid-Tact Osteocalcin Elisa Kit is highly specific. It measures both the intact human osteocalcin and the major (1-43) fragment. The assay is a sandwich EIA which employs two monoclonal antibodies. One antibody (1-19) is immobilized in the wells and the second antibody (30-40) is biotinylated. The assay is highly sensitive (0.5ng/ml) and requires only a 25 microliter sample. All the necessary reagents, a 96-well strip plate, and a complete 3½ hour protocol are included with the kit.
Mid-Tact Human Osteocalcin EIA Kit
Specifications:
Sample Size: 25ul
Assay Time: 3.5 hours
Sensitivity: 0.5 ng/ml
Range: 1.0-50 ng/ml
Intraassay Variation: 7%
Interassay Variation: 10%
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