Osteocalcin (5800 daltons) is a specific product of the osteoblast. It is primarily deposited into the ECM of bone and only a small amount of newly synthesized osteocalcin is released directly into the circulation. Studies suggest that there are various forms of osteocalcin in the circulation and that different antibodies detect both intact and fragments of osteocalcin. The physiological significance of osteocalcin fragments is unclear but they may be derived from resorption, osteoblastic synthesis, systemic catabolism or all of these. Since serum proteases may also degrade the intact osteocalcin, it is recommended that blood samples be processed quickly in the presence of protease inhibitors.
The Intact Osteocalcin EIA measures only intact osteocalcin which is synthesized de novo by the osteoblast and it eliminates any interference by circulating fragments. The assay is a sandwich EIA which employs two monoclonal antibodies directed towards the amino-and the carboxy-terminal regions of the human osteocalcin. It recognizes only intact osteocalcin, requiring the full 49 residue protein for detection. This assay reacts with human, bovine, monkey and dog osteocalcin.
The assay is highly sensitive (Range: 1-50ng/ml) and requires only a 25ul sample. All the necessary reagents, a 96-well strip plate, and a complete 3½ hour protocol are included with the kit.
产品家族搜索-Family Search：